Published: November 12th, 2019 Last Modified: November 22nd, 2019
Already purified pfu-sso7d? Got a lifetimes supply of Mashup-RT in the freezer? Still hungry for more??? Don’t worry, I have you all covered. With gene synthesis rates plummeting we can move past purifying the low hanging fruit and get into some niche, but useful, enzymes.
Check out the Plasmid List to see what’s on offer!
I am introducing two enzymes to the lineup: DpnI and Murine RNase inhibitor (MRI).
If you spend more than a couple hundred dollars a month on either enzyme it is certainly worth the effort to purify your own. DpnI is extremely useful in cloning/PCR heavy labs for digesting plasmid DNA after a PCR reaction is complete. MRI is used in reverse transcription and any reaction where you suspect possible RNase contamination. Purifying your own can not only save you money, but you can find new applications for the enzymes where it was cost prohibitive before.
Purification protocols are coming, beta testers please apply!
Thank you that you continue to work on more lab routine tools.
Can I have the plasmids for DpnI and MRI? I would like do the purification test– of course for our daily usage.