Cheat Sheets / Calculators

Published: November 23rd, 2019   Last Modified: November 29th, 2019

LinkNotesCredit
Plasmid Prep Cheatsheet.docx
.pdf
Silica/DEAE/Phenol plasmid prep methods, meant for use with generic and name brand columns.PJ
Silica Column Cheat Sheet.pptx
.pdf
Agarose gel purification, PCR enzymatic product purification with silica columns, can use name brand or generic.PJ
PCR Mastermix Calculator.xlsxEdit volumes of reagents in 1x multiplier column, set multiplier for your mastermix, excel does the rest. Primers can be added to primer list.
Best printed with sticky label maker.
PJ
Gel Buffer Calculator.xlsxUseful for lots of gel extractions, how much buffer do you need for a certain amount of gel? Print it out and stick it up.PJ
Gibson Assembly CalculatorBarrick Lab LinkCalculates what volume of DNA, of a certain length and concentration, is needed to get your usual 1:3:3 or 1:1:1 ratios for cloning reactions, Gibson/TEDA etc.Ben from Barrick Lab

Plenty more to come, just making things pretty for you guys.